Fig. 3. Delivery of pDNA/IL nanocomplexes, and plasmid DNA/IL nanocomplexes subjected to ultrasonic shear stress for different time periods to COS7 cells. (a) pDNA/IL nanocomplexes; (b) pDNA/IL nanocomplexes were complexed with 2 μL lipofectamine (LA). (c) pDNA/IL nanocomplexes were subjected to 10 min ultrasonic shear stress and complexed with LA; (d) pDNA/IL nanocomplexes were subjected to 20 min ultrasonic shear stress and complexed with LA; (e) pDNA/IL nanocomplexes were subjected to 30 min ultrasonic shear stress and complexed with LA; (f) pDNA/IL nanocomplexes were subjected to 40 min ultrasonic shear stress and complexed with LA; (g) pDNA/IL nanocomplexes were subjected to 60 min ultrasonic shear stress and complexed with LA; (h) pDNA/IL nanocomplexes were subjected to 90 min ultrasonic shear stress and complexed with LA; (i) pDNA/IL nanocomplexes were subjected to 120 min ultrasonic shear stress and complexed with LA; scale bar: 100 μm.
