Fig. 4. miR-221 reversed HOTAIR-mediated promotion of apoptosis in MPP+-treated SH-SY5Y cells. Cell viability (A and B), levels of Bcl-2 and Bax protein (C and D) and apoptosis (E and F) were detected in SH-SY5Y cells co-transfected with HOTAIR + miR-221, HOTAIR + miR-NC, si-HOTAIR + anti-miR-221 or si-HOTAIR + anti-miR-NC after treatment with 1 mM MPP+ for 24 h by MTT, western blot and flow cytometry, respectively. *P < 0.05, **P < 0.01, ***P < 0.001.
