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. 2022 Apr 28;2022:9716045. doi: 10.1155/2022/9716045

Table 1.

Design of reverse transcription primers for qPCR detection of SCN5A in peripheral blood.

Primer name Primer sequence
SCN5A mutation site pre-qPCR F 5′-CCAGACAGAGGGAGACTTGC-3′
SCN5A mutation site pre-qPCR R 3′-CTGGAGTCCACAGCTGCATA-5′
qPCR after SCN5A mutation site F 5′-CGCCTACGTGATGAGTGAGA-3′
qPCR after SCN5A mutation site R 3′-GTCGGCGAGATCTTCACTGT-5′
hGAPDH F 5′-CAAGGTCATCCATGACAACTTTG-3′
hGAPDH R 3′-GTCCACCACCCTGTTGCTGTAG-5′

F as forward and R as reverse.