Effect of lack of each essential component in the culture on the growth and BS production.
| Lack of the component | Growth | BS production (g L−1) after 96 h |
|---|---|---|
| MgSO4 | The lack of significant growth | 0 |
| Oil | Low growth during the early hours due to the presence of yeast extract and then stop growing to day 4 | 0 |
| Yeast extract | Up to 6 hours there is no growth. After the matching bacteria with environment, growth begins on oil | 3.4 |
| NaNO3 | Slowdown in growth rate | 3.7 |
| KH2PO4 | Slowdown in growth rate | 4.4 |