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. 2021 Aug 14;12(3):1288–1304. doi: 10.1016/j.apsb.2021.08.015

Figure 2.

Figure 2

Z-GP-DAVLBH induces apoptosis in osteosarcoma cells. (A) and (B) Osteosarcoma cells (SJSA-1 and 143B) were (A) exposed to various concentrations of Z-GP-DAVLBH for 48 h, or (B) treated with Z-GP-DAVLBH for the indicated times. Apoptosis was examined with an Annexin V-FITC apoptosis detection kit. Representative flow cytometry plots and quantification of apoptotic cells. (C)–(E) Osteosarcoma cells (SJSA-1 and 143B) were (C) treated with Z-GP-DAVLBH for the indicated times or (D) incubated with gradient concentrations of Z-GP-DAVLBH for 48 h. Mitochondrial membrane potential (MMP) was assessed with a JC-1 kit. (E) Quantification of JC-1 polymer fluorescence of (D) is shown. (F) The expression of PARP, cleaved PARP, caspase-3, cleaved caspase-3, caspase 9, and cleaved caspase-9 in SJSA-1 and 143B cells were determined by Western blotting analysis. Representative blots are shown. Data are presented as mean±SEM, n = 3; ∗P<0.05, ∗∗P<0.01, and ∗∗∗P<0.001 vs. the untreated (0 nmol/L, 0.1% DMSO) group.