TABLE 2.
Inhibition of replication of avian influenza A viruses in MDCK cells by RWJ-270201, zanamivir, and oseltamivir carboxylate
| NA subtype | Virusb | Mean EC50 ± SE (μM)a
|
||
|---|---|---|---|---|
| RWJ-270201 | Zanamivir | Oseltamivir carboxylate | ||
| N1 | A/teal/Hong Kong/W312/97 (H6N1) | 5.0 ± 1.8 | 9.4 ± 0.7 | 8.1 ± 1.6 |
| A/duck/Alberta/35/76 (H1N1) | 1.1 ± 0.4 | 11.3 ± 2.4 | 11.9 ± 0.5 | |
| N2 | A/chicken/NY/13307-3/95 (H7N2) | 1.1 ± 0.3 | 6.9 ± 0.8 | 1.0 ± 0.3 |
| A/turkey/Wisconsin/66 (H9N2) | 0.9 ± 0.4 | 19.7 ± 0.9 | 1.0 ± 0.1 | |
| N3 | A/duck/Singapore/3/97 (H5N3) | 1.0 ± 0.5 | 10.3 ± 0.2 | 1.6 ± 0.5 |
| A/duck/Germany/1215/73 (H2N3) | 1.4 ± 0.2 | 32.3 ± 1.5 | 6.8 ± 1.2 | |
| N4 | A/red knot/DE/254/94 (H8N4) | 8.5 ± 2.5 | 17.5 ± 0.5 | 17.9 ± 0.1 |
| A/turkey/Ontario/6118/68 (H8N4) | 2.6 ± 1.4 | 12.0 ± 1.8 | 6.2 ± 2.2 | |
| N5 | A/mallard duck/Astrakhan/263/82 (H14N5) | 11.8 ± 0.5 | 12.0 ± 0.3 | 13.0 ± 1.0 |
| A/shearwater/Australia/1/72 (H6N5) | 2.7 ± 0.9 | 19.3 ± 3.0 | 3.5 ± 1.4 | |
| N6 | A/shorebird/DE/224/97 (H13N6) | 11.0 ± 0.8 | 11.2 ± 0.6 | 10.2 ± 0.6 |
| A/duck/Czechoslovakia/56 (H4N6) | 8.7 ± 1.7 | 42.0 ± 1.5 | 10.7 ± 0.9 | |
| N7 | A/chicken/Jena/1816/87 (H7N7) | 0.5 ± 0.1 | 12.3 ± 1.5 | 4.7 ± 0.9 |
| A/duck/Germany/“N”/49 (H10N7) | 1.7 ± 0.5 | 27.0 ± 1.2 | 3.8 ± 0.8 | |
| N8 | A/duck/Hong Kong/Y264/97 (H4N8) | 2.3 ± 0.8 | 4.0 ± 1.0 | 3.5 ± 2.5 |
| A/quail/Italy/1117/65 (H10N8) | 2.7 ± 0.4 | 55.0 ± 8.8 | 1.0 ± 0.1 | |
| N9 | A/duck/Hong Kong/P50/97 (H11N9) | 7.9 ± 1.7 | 10.8 ± 0.6 | 3.7 ± 3.3 |
| A/duck/Memphis/546/74 (H11N9) | 11.7 ± 1.8 | 58.3 ± 1.7 | 42.0 ± 1.5 | |
| Range (N1–N9) | Range of values | 0.5–11.8 | 4.0–58.3 | 1.0–42.0 |
a
Antiviral activity was assessed in MDCK cells by a modified microneutralization assay followed by ELISA. Values are means ± SEs for at least three independent determinations.
b
The second strain in each pair is the reference strain of influenza A virus used for the antigenic subtyping of the NA of the new influenza isolates.