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. 2022 May 5;12:7372. doi: 10.1038/s41598-022-11025-x

Figure 2.

Figure 2

Experimental timeline and viral confirmation of shScramble and shCHOP injections. (a) rTg4510 mice received hippocampal injections of AAV9 shScramble or shCHOP containing EGFP tag at 8.5 months of age. (b) Representative images of EGFP and CHOP signal in hippocampal regions from mice expressing AAV9-shScramble [n = 7] or AAV9-shCHOP [n = 9]. (c) Sections expressing EGFP were analyzed for the integrated density of GADD153 (CHOP protein) staining in the hippocampus of rTg4510 mice injected as described above. Colocalization analysis was performed by (d) Mander’s coefficient (Coef.) to estimate the fraction of CHOP being colocalized with GFP and (e) Pearson’s Coef. to measure the co-occurrence of GFP/CHOP intensities. (f) Sections were also analyzed for the integrated density of GFP fluorescence in the hippocampus. (g) A correlation between viral transduction (GFP) and CHOP was performed. Data are represented in standard error of the mean (± SEM) and analyzed by unpaired Student’s t-test. Statistical significance is shown by *p = 0.0384. Scale bar represents 20 µm. CHOP CCAAT-enhancer binding protein homologous protein, GFP green fluorescent protein, HPC hippocampus.