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. 2022 May 5;12:7324. doi: 10.1038/s41598-022-11048-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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EB-FPDK11 and EB-AMDK19 facilitate intestinal epithelial barrier function maintenance from DNCB-induced colon injury. (A) H&E staining of the large intestinal tissues from DNCB-induced AD-like Nc/Nga mice. The yellow lines indicate the villi length. Scale bar, 100 µm. (B) Large intestine villi length was determined by measuring vertically well-oriented crypt villi from H&E stained sections. (C) Alcian blue PAS staining of the large intestinal tissues, the red arrows indicate positively stained goblet cells. (D) Quantitative analysis of the number of goblet cells in the crypt of the colon. (E) The expression of ZO-1 proteins in the colon tissue were analyzed using immunohistochemistry staining. Scale bar, 50 µm. (F) Relative expression of ZO-1 mRNA expression in the colon tissue was determined by quantitative PCR. (G–I) Transcript levels of Th2 related cytokine genes, mIL-13 (G), mIL-6 (H), and mIL-10 (I) were determined by quantitative PCR. Data are presented as mean ± SEM of changes in values. *P < 0.05, **P < 0.01, ***P < 0.001; *versus normal group; ^#versus DNCB group; ^$versus DEX group; ^&versus BAA-835 group.