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. 2022 May 5;13:2488. doi: 10.1038/s41467-022-30239-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Live-cell imaging was performed in caulonemal apical cells of P. patens, expressing mCherry-tubulin and one of the following TPX2 proteins endogenously tagged with a fluorophore: a TPX2-1-Citrine; b SunTag-TPX2-1; c TPX2-2-mNeonGreen; d mNeonGreen-TPX2-4; e TPX2-5-mNeonGreen. The SunTag-TPX2-1 line also expressed scFv-GCN-sfGFP under a β-estradiol-inducible promoter. Localization was observed in 2 independent experiments with similar results. Arrowheads indicate the fluorescent tag-based signals, whereas other ellipsoidal signals outside the spindle/nucleus represent autofluorescent chloroplasts. Bars, 10 µm.