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. 2022 May 5;13:2483. doi: 10.1038/s41467-022-30126-9

Fig. 1. Combinatorial CRISPR screen of the SLC25 family transporters across four media conditions.

Fig. 1

a Schematic overview of the combinatorial CRISPR strategy. b SLC25-Papi library construction. c Growth rate of the pooled CRISPR screen. The batch 2 growth rate was shown and representative of the two batches. d positive control pair BCL2L1 and MCL1. Data shown are log2 fold changes from day 15 in the glucose condition. Gray, control-control pair; pink, BCL2L1 x Ctrl or Ctrl x BCL2L1; blue, MCL1 x Ctrl or Ctrl x MCL1; and purple, the double knockouts of BCL2L1 x MCL1 or MCL1xBCL2L1. The black lines indicate the median and the red line indicates the expected value for the double knockout based on the sum of the single knockouts when there is no genetic interaction. Statistical significance was calculated using two-tailed t test. Source data are provided as a Source Data file.