Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 5;13:2483. doi: 10.1038/s41467-022-30126-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Heatmap shows the π-score for pairwise genetic interaction. The values are z-transformed to compare across media conditions, as described in the methods. For comparison, a positive control pair (BCL2L1 x MCL1) that are always detrimental when jointly knocked out and another pair (UCP1 x SLC25A31) that do not interact are shown. The rows and columns are not clustered. b LFC values from the CRISPR screen are shown to illustrate the strongest synthetic sick genetic interaction observed from the CRISPR screen, SLC25A5 and SLC25A6 loss in the galactose condition. The red line indicates the expected LFC, based on an additive model, if there were no genetic interaction. The black line indicates the actual median LFC value. c Follow-up validation of the genetic interaction between SLC25A5 and SLC25A6 in the galactose (p_ctrl/A5KO = 0.59, p_ctrl/A6KO = 0.66, p_A5KO/DKO = 2.11 × 10⁻⁷, p_A6KO/DKO = 1.93 × 10⁻⁸). The dotted line indicates the expected growth fitness, based on an additive model, if there were no specific genetic interaction (n = 3). d The cartoon illustrates the function of ANTs in supporting bioenergetics. e Transcript levels of the four ANTs in K562 cells (data plotted from the published PBS vehicle treated K562 cells RNA-seq dataset GSE74999) (n = 3). f qPCR of SLC25A4 mRNA level in SLC25A5 KO and SLC25A5 and SLC25A6 DKO cells (n = 3). g Follow-up validation of the genetic interaction between SLC25A37 and SLC25A39 in the glucose (p_ctrl/A37KO = 1.04×10^-5, p_ctrl/A39KO = 3.08×10^-6, p_A37KO/DKO = 0.56, p_A39KO/DKO = 0.0092) and antimycin (p_ctrl/A37KO = 0.00074, p_ctrl/A39KO = 0.0498, p_A37KO/DKO = 5.54×10^-5, p_A39KO/DKO = 0.030) conditions (n = 6). Statistical significance was calculated using two-tailed t test. Significance level were indicated as *** p < 0.001, ** p < 0.01, * p < 0.05 and n.s. p > 0.05. Data are expressed as mean ± SD. Source data are provided as a Source Data file.