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. 2022 May 5;13:2460. doi: 10.1038/s41467-022-29978-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Location of B1-8i/k^−/−/Blimp1^GFP/Cdt1m^KO2 B cells in drLN 6 d after immunization. Antigen-specific B cells in the G0/G1 phase of cell cycle (red) inside the GC (dashed line) and in the follicle (F) close to the SCS (arrow heads). Interfollicular region (open triangle). Blimp-1⁺ PCs are eGFP (green). Hoechst33342-labeled naïve T cells (blue). Scale bar: 100 μm. Representative image of 3 lymph nodes. b Gating of eGFP⁺ B_EM in drLN and B_CM and distLN 8 d after immunization of recipients of NP-specific Cγ1Cre QM mTmG B cells. c Kinetic of eGFP⁺ B cell appearance in drLN and distLN. Data merged from two independent experiments (n = 2–3). Each datapoint represents one animal. d Memory B cell typical markers on B_EM and B_CM in drLN and distLN. e drLN of recipient of QM Cγ1Cre mTmG cells 8 d after immunization. T zone (T). f Enlargement of box in e showing the eGFP⁺ B_EM close to the subcapsular sinus, g Ki-67 expression in B_EM, h same area showing B_EM location below the LYVE1⁺ ER-TR7^-ve SCS floor endothelium and inside the SCS (arrowheads). Image is a representative of three lymph nodes.