Skip to main content
. 2022 May 5;13:2460. doi: 10.1038/s41467-022-29978-y

Fig. 3. CCR7-dependent migration of memory B cells.

Fig. 3

a CCR7 expression on different lymphocyte subsets measured by flow cytometry. Each symbol represents one animal. Two-tailed Mann-Whitney test, *p = 0.0286. b drLN from a heterozygous Ccl21tdTom/wt reporter mouse 8 d after foot immunization. The SCS ceiling endothelium is missing. Images show dTomato expression in red, and IgD (blue), CD169 (turquoise) and Lyve-1 (green)-specific antibody staining. Arrow heads: Ccl21 expression in SCS. Enlarged box in shows Ccl21a expression under and in the SCS floor endothelium. Image is a representative from 4 lymph nodes. c GC B cells, plasma cells (PC), BEM from drLN 8 d after foot immunization of recipients of a mix of QM mT CCR7+/+ and QM eYFP CCR7−/− B cells. BCM in blood, distLN, spleen, and bone marrow (BM). Two- tailed Wilcoxon matched-pairs test. d Ratio of CCR7−/− to CCR7+/+ QM B cells in different tissues. Two-tailed unpaired t test. Each symbol represents one animal. Data are from two independent experiments with 5–6 mice each.