Skip to main content
. 2022 May 5;13:2469. doi: 10.1038/s41467-022-30196-9

Fig. 5. Positive and negative regulators of the MAPK pathway exhibit synergistic dependencies in NRAS-mutant melanoma.

Fig. 5

a Manhattan plot of FDRs corresponding to GEMINI synergy scores and color-coded by the LFC of dependencies for paralog pair knockouts in IPC298. The dotted line represents the FDR of 1e-3. b Scatter plot of GEMINI synergy score and LFC of dual knockout in IPC298. Curated paralog pairs of the MAPK pathway are annotated by red dots. Pan-essential paralog pairs or genes are annotated by blue or yellow dots, respectively. c LFC of single or combinatorial gene knockouts of paralog pairs associated with MAPK signaling pathway in IPC298 (n = 3 biological replicates; 6 independent sgRNAs per gene and 18 independent sgRNA combinations per gene pair). The centerline, lower hinge, and upper hinge correspond to the 50th, 25th, and 75th percentiles, respectively. The upper and lower whiskers extend from the upper and lower hinges to the largest and smallest values no further than 1.5 * IQR (interquartile range). Source data are provided in the Source Data File.