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. 2022 Apr 22;13:868892. doi: 10.3389/fimmu.2022.868892

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Copyright © 2022 Zhao, Yao, Chen, He, Xie, Zhang, Lin, Chen, Xie and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of differentially expressed lncRNAs and m⁶A modification levels of lncRNAs in normal livers and ALV-J induced tumor livers using RT-qPCR and MeRIP-qPCR. (A) The relative lncRNA expression level of CMPK2, HDAC9, MINDY4B, ATOH8, and LOC100859478 in ALV-J induced tumor livers (positive) and normal livers (negative) was detected by RT-qPCR. (B) The m⁶A level of HDAC9, MINDY4B, and SOX7 was validated by MeRIP-qPCR. Expression levels were normalized by GAPDH. (C) The lncRNA fold change of CMPK2, HDAC9, MINDY4B, ATOH8, and LOC100859478 in ALV-J induced tumor livers (positive) and normal livers (negative) in RNA-Seq results. (D) The m⁶A level of HDAC9, MINDY4B, and SOX7 in MeRIP-seq results. Data are presented as mean ± SEM. **P < 0.05; *P < 0.01. Negative represents chickens without ALV-J infection (normal livers); Positive represents chickens with ALV-J infection (tumor livers).