Fig. 6. Western blot confirms PsaF is lost in PSI-SMALPs. In the far-left lane, PsaF is shown to dissociate from Chroococcidiopsis TS-821 PSI monomer when extracted with SMA 1440. In the second lane from left, when extracted with DDM PSI from this organism retains both a tetrameric oligomeric state and PsaF. The third lane from left shows PsaF was found at the top of the sucrose density gradient, following solubilization. The far-right lane shows PsaF is missing in the trimeric PSI-SMALP.