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. 2022 May 5;12:7334. doi: 10.1038/s41598-022-10925-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cell-surface ZNT1 expression is enhanced by Dox-induced expression of mouse Zip4 and Zip5 and human ZIP4 and ZIP5. (A,B) Transiently expressed Zip4/Zip5 and ZIP4/ZIP5 enhanced cell-surface ZNT1 expression. U2OS cells were transiently transfected with an IRES-GFP expression plasmid harboring Zip4-HA or Zip5-HA cDNA (A) or ZIP4-HA or ZIP5-HA cDNA (B), and transfected cells were identified by their GFP fluorescence. DAPI staining is also shown in the merged images, where line-profile analysis was used to semi-quantitatively determine relative fluorescence intensity (white arrows); the graphs on the right show the relative fluorescence intensity along each arrow. Each experiment was performed at least thrice, and representative results from independent experiments are shown.