Fig. 4.

Effect of MLK on the apoptotic pathway p-AKT1/cleaved caspase-3 in GM-treated rats. (A) Western blots for p-AKT1 and cleaved caspase-3. (B, C) Graphical presentations for the changes in protein expression of p-AKT1 and cleaved caspase-3, respectively. Administration of MLK with GM significantly attenuated the apoptotic effect of GM through enhancing p-AKT1 protein expression, while reducing that of cleaved caspase-3. Dots represent individual values, while bars represent mean ± SD (n = 3–4). Comparisons were made using one-way ANOVA followed by Tukey’s post-hoc test. ^a Significantly different from control group at p < 0.05, ^b Significantly different from GM group at p < 0.05.