REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Actin	Sigma-Aldrich	Cat# A4700; RRID: AB_476730
β-catenin	Sigma-Aldrich	Cat# C7207; RRID: AB_476865
GST-Tag	Proteintech	Cat# 66001-2-Ig; RRID: AB_2881488
TELO2	Proteintech	Cat# 15975-1-AP; RRID: AB_2203337
TTI1	Proteintech	Cat# 22381-1-AP; RRID: AB_2879094
Axin2	Cell Signaling Technology	Cat# 2151; RRID: AB_2062432
Cyclin D1	Cell Signaling Technology	Cat# 2926; RRID: AB_2070400
mTOR	Cell Signaling Technology	Cat# 2972; RRID: AB_330978
ATM	Cell Signaling Technology	Cat# 2873; RRID: AB_2062659
ATR	Cell Signaling Technology	Cat# 13934; RRID: AB_2798347
DNA-PK	Cell Signaling Technology	Cat# 12311; RRID: AB_2797881
Phospho-AKT (Ser473)	Cell Signaling Technology	Cat# 4060; RRID: AB_2315049
AKT	Cell Signaling Technology	Cat# 4691; RRID: AB_915783
Phospho-p70 S6 kinase (Thr389)	Cell Signaling Technology	Cat# 9206; RRID: AB_2285392
p70 S6 Kinase	Cell Signaling Technology	Cat# 9202; RRID: AB_331676
DDDDK-tag (FLAG-tag)	Medical & Biological Laboratories	Cat# M185-3L; RRID: AB_11123930
Bacterial and virus strains
ECOS Competent E. coli DH5α	FUJIFILM	Cat# 310-06236
Chemicals, peptides, and recombinant proteins
Wnt-3A	R&D systems	Cat# 5036-WN-010
Ivermectin	Sigma-Aldrich	I8898; CAS: 70288-86-7
MG132	Sigma-Aldrich	474791; CAS: 133407-82-6
6-bromoindirubin-3’-oxime	Sigma-Aldrich	B1686; CAS: 667463-62-9
Torin2	Selleck	S2817; CAS: 1223001-51-1
Rapamycin	Sigma-Aldrich	R0395; CAS: 53123-88-9
Lactacystin	FUJIFILM Wako Pure Chemical Corporation	333-43681; CAS: 133343-34-7
XAV-939	Tocris Bioscience	3748; CAS: 284028-89-3
FG beads (alkyne beads)	Tamagawa Seiki	TAS8848 N1161
IVM B1a-SNIPER 1	This article	N/A
IVM B1a-SNIPER 2	This article	N/A
IVM B1a-SNIPER 3	This article	N/A
IVM B1a-SNIPER 4	This article	N/A
Lipofectamine RNAiMAX Transfection Reagent	Thermo Fisher Scientific	Cat# 13778030
TransIT-X2 Dynamic Delivery System	Mirus Bio	Cat# MIR6003
TransIT-LT1 Transfection Reagent	Mirus Bio	Cat# MIR2300
Critical commercial assays
Dual-Luciferase Reporter Assay System	Promega	Cat# E1960
Amersham ECL Western Blotting Detection Reagent	GE Healthcare	Cat# RPN2209
Experimental models: Cell lines
Human: HEK293 cells	RIKEN BRC	Cat# RCB1637
Human: DLD-1 cells	ATCC	Cat# CCL-221
Human: HT-29 cells	ATCC	Cat# HTB-38
Experimental models: Organisms/Strains
Zebrafish: wild-type strain RIKEN	The National Bioresource Project of Japan	RIKEN WT (RW)
Oligonucleotides
GeneSolution siRNA Hs_TELO2_1 (siTELO2 #1)	Qiagen	Cat# SI04152904
GeneSolution siRNA Hs_TELO2_3 (siTELO2 #3)	Qiagen	Cat# SI04365249
GeneSolution siRNA Hs_KIAA0683_3 (siTELO2 #4)	Qiagen	Cat# SI00454902
MISSION siRNA Universal Negative Control#1	Merck	Cat# SIC001
Primers for the construction of TELO2 mutant expression vectors, see Data S1	Eurofins Scientific	N/A
Recombinant DNA
Super 8x TOPFlash	Addgene	Cat# 12456 (Veeman et al., 2003)
pRL-SV40	Promega	Cat# E2231
p3xFLAG-CMV10-hTel2	Addgene	Cat# 30214 (Kaizuka et al., 2010)
p3xFLAG-CMV10-hTel2 K749T	This article	N/A
pGEX6p1-TELO2 CTD	This article	N/A
pGEX6p1-TELO2 CTD Δ5	This article	N/A
pGEX6p1-TELO2 CTD Δ6	This article	N/A
pGEX6p1-TELO2 CTD Δ7	This article	N/A
pGEX6p1-TELO2 K749T	This article	N/A
pGEX6p1-TELO2 E753A	This article	N/A
pGEX6p1-TELO2 R759G	This article	N/A
pGEX6p1-TELO2 H761L	This article	N/A
pGEX6p1-TELO2 D763A	This article	N/A
pGEX6p1-TELO2 R767G	This article	N/A
Software and algorithms
ImageJ	National Institutes of Health, USA	ver. 1.52a; RRID:SCR_003070; https://imagej.nih.gov/ij/
BellCurve for Excel	Social Survey Research Information	Ver. 3.22; RRID:SCR_017294; https://bellcurve.jp/ex/