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. 2021 Dec 22;29(4):2604–2612. doi: 10.1016/j.sjbs.2021.12.042

Table 2.

Protein profile alternation in control and Cr treated seed of S1 (A) and S2 (B) varieties; where fold change 1 = difference between control and 200 ppm and Fold change 2 = difference between control and 400 ppm, plus sign indicate an increase and minus sign indicate a decrease in expression as compared to reference gel.

For S1 (A)
Expression of total protein concentration load per sample (10 µg)
Sr No. Mol.Wt (kDa) Rf value Control 200 ppm 400 ppm Fold change (1) Fold change (2)
1 122 0.045 0.5 −0.5 −0.5
2 116 0.054 0.97 +0.97 +0.97
3 112 0.060 1.50 +1.50
4 66 0.210 1.21 +1.21
5 60 0.249 0.49 0.89 0.82 +1.81 +1.67
6 55 0.275 1.4 1.03 +1.4 +1.03
7 48 0.323 1.59 +1.59
8 47 0.332 0.53 1.24 +2.33 −0.53
9 42 0.404 0.49 1.40 1.55 +2.85 +3.16
10 27 0.722 1.26 +1.26
11 20 0.859 7.48 1.61 2.31 − 4.64 −3.23
12 12 0.955 0.51 1.23 +2.41 −0.51
For S2 (B) Expression of total protein concentration load per sample (10 µg)

Sr No. Mol.Wt (kDa) Rf value Control 200 ppm 400 ppm Fold change (1) Fold change (2)

1 120 0.048 1.73 1.21 1.34 −1.42 −1.29
2 68 0.203 1.18 +1.18
3 59 0.251 1.26 0.72 −1.75 +1.38
4 57 0.256 1.31 0.00 +1.31
5 49 0.316 1.51 +1.55
6 47 0.331 1.47 1.43 −1.47 +1.02
7 42 0.403 1.51 1.56 1.57 +0.96 −1.03
8 26 0.719 1.59 +1.59
9 21 0.848 2.38 2.22 2.87 −1.07 −1.20
10 13 0.940 1.64 1.49 −1.64 +1.1