Table 2.
The effect of different nano amino zinc concentrations on mitosis division and chromosomal aberrations under different time treatments.
Types | Control | Concentration of AZ NPs and time of treatments (Mean + SEM) |
||||||||
---|---|---|---|---|---|---|---|---|---|---|
After 8 hrs |
After 16 hrs |
After 24 hrs |
||||||||
50 ppm | 100 ppm | 150 ppm | 50 ppm | 100 ppm | 150 ppm | 50 ppm | 100 ppm | 150 ppm | ||
NOC | 1838 ± 4.4a | 1999.6 ± 6.3a | 1804 ± 3.6a | 1838.6 ± 5.2a | 1996 ± 5.3a | 1642 ± 5.1a | 1716.7 ± 6.2a | 1908.3 ± 5.9a | 2047.3 ± 6.9a | 1948.6 ± 4.2a |
NDC | 1630 ± 3.2b | 1483.5 ± 4.6b | 1302.5 ± 4.4b | 1206.5 ± 6.5b | 953.5 ± 4.9b | 901 ± 4.8b | 895 ± 5.3b | 860 ± 3.8b | 819 ± 4.6b | 700.0 ± 5.2b |
Prophase | 1054 ± 5.3c | 962.67 ± 4.3c | 845.67 ± 4.8c | 785.67 ± 4.2c | 618.3 ± 4.2c | 584.33 ± 4.6c | 575.0 ± 4.9c | 558.0 ± 4.2c | 532.33 ± 4.1c | 454.0 ± 3.9c |
Metaphase | 232.5 ± 2.6e | 192.5 ± 2.9e | 169.0 ± 2.9e | 157.5 ± 3.6e | 124.5 ± 3.6f | 117.5 ± 2.4f | 117.0 ± 2.3e | 112.5 ± 2.9f | 109.5 ± 1.6e | 95.5 ± 2.3f |
Anaphase | 262.0 ± 2.9d | 220.67 ± 3.6d | 193.67 ± 3.3d | 181.0 ± 4.2d | 141.0 ± 3.4d | 132.33 ± 2.6e | 131.67 ± 2.8d | 130 ± 2.6e | 121.67 ± 2.3e | 106 ± 2.5e |
Telophase | 75.67 ± 1.7g | 101.67 ± 1.9g | 91.67 ± 2.2g | 82.33 ± 1.8g | 65.0 ± 1.9g | 62.0 ± 1.1g | 61.0 ± 1.4f | 60.0 ± 1.5g | 57.0 ± 1.4f | 50.0 ± 1.8g |
M.I. | 88.0 ± 1.4f | 74.33 ± 2.2h | 71.66 ± 2.4h | 66.33 ± 3.2h | 48.3 ± 1.1h | 53.33 ± 1.3g | 50.33 ± 1.3g | 43.66 ± 1.6h | 40.0 ± 1.1g | 35.3 ± 1.1h |
NAC | 20.33 ± 1.1h | 131.33 ± 3.2f | 156.33 ± 2.8f | 142.33 ± 3.6f | 129.6 ± 2.3e | 145 ± 2.3d | 140.66 ± 3.6d | 155 ± 3.3d | 183.66 ± 2.2d | 239.6 ± 2.7d |
% of aberration | 1.33 ± 0.3i | 8 ± 0.44i | 11 ± 0.58i | 11 ± 0.44i | 13.3 ± 0.52i | 15.6 ± 0.4h | 16.33 ± 0.5h | 18 ± 0.51i | 23.33 ± 0.9h | 34 ± 1.5h |
LSD | 5.16 | 4.25 | 4.27 | 5.14 | 4.87 | 10.8 | 9.96 | 6.57 | 15.2 | 4.92 |
*NOC: Number of Observed Cells (total cells); NDC: Number of Divided Cells (in different mitosis stages); M.I.: Mitotic Index; NAC: Number of Abnormal Cells (total abnormal cells); % of aberration: the percentage of aberrations in cells.