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| Run | Item | LLOQ (0.1 ng mL−1) | Low QC (0.25 ng mL−1) | Medium QC (5 ng mL−1) | High QC (150 ng mL−1) | Dilution (400 ng mL−1) |
|---|---|---|---|---|---|---|
| Within-run (n = 5) | Mean ± SD | 0.109 ± 0.003 | 0.263 ± 0.006 | 4.95 ± 0.352 | 146 ± 2.98 | 387 ± 11.4 |
| Precision (RSD%) | 2.8 | 2.3 | 7.1 | 2.0 | 2.9 | |
| Accuracy (%) | 109.0 | 105.3 | 99.1 | 97.0 | 96.8 | |
| Between-run (n = 15) | Mean ± SD | 0.112 ± 0.005 | 0.269 ± 0.016 | 5.27 ± 0.487 | 142 ± 3.73 | — |
| Precision (RSD%) | 4.5 | 5.9 | 9.2 | 2.6 | — | |
| Accuracy (%) | 112.0 | 107.6 | 105.4 | 94.7 | — |
