Absorption (λmaxAb) and emission (λmaxFl) maxima, extinction coefficients (ε) and fluorescence quantum yields (ΦF) of RD dye and IRD-CLB and 5-CLB conjugates measured at 25 °C in 10 mM phosphate buffer pH 7.5 (PB) and cell medium pH 7.5 (CM).
| Dye/conjugate | PB : ACN (4 : 1, v/v) | CM : ACN (4 : 1, v/v) | |||||
|---|---|---|---|---|---|---|---|
| λ max Ab (nm) | ε (M−1 × cm−1) | λ max Fl (nm) | Φ F (%) | λ max Ab (nm) | λ max Fl (nm) | Φ F (%) | |
| RD | 555 | 57 000 | 643 | 9.9 ± 0.8 | 526 | 626 | 2.6 ± 0.1 |
| IRD-CLB | 775 | 160 000 | 795 | 2.9 ± 0.3 | 785 | 805 | 6.3 ± 0.5 |
| 5-CLB | 783 | 160 000 | 796 | 1.5 ± 0.2 | 787 | 805 | 5.9 ± 0.4 |