Table 3.
Amino acid composition of the eluted protein band (22 kDa) “attacin-like” of S. littoralis 5th instar larvae and its comparison with other attacin AMPs from other lepidopterous insects.
| Amino acid | Band (22 kDa) “attacin-like” | Attacin of Spodoptera exigua | Attacin of Bombyx mori | Attacin of Manduca Sexta |
|---|---|---|---|---|
| Ala (A) | 8.0 | 6.3 | 11.2 | 9.3 |
| Arg (R) | 6.6 | 4.3 | 4.7 | 3.1 |
| Asn (N) | 9.1 | 9.1 | 8.9 | 7.6 |
| Asp (D) | 8.5 | 5.5 | 5.1 | 9.3 |
| Cys (C) | 0.0 | 0.0 | 0.9 | 0.1 |
| Gln (Q) | 3.5 | 3.4 | 1.4 | 0.0 |
| Glu (E) | 3.6 | 3.9 | 0.9 | 0.9 |
| Gly (G) | 10.2 | 10.6 | 10.3 | 9.3 |
| His (H) | 3.9 | 3.2 | 2.8 | 3.1 |
| Ile (I) | 2.7 | 1.2 | 2.3 | 2.2 |
| Leu (L) | 7.9 | 10.2 | 9.8 | 8.4 |
| Lys (K) | 4.0 | 3.9 | 5.6 | 6.7 |
| Met (M) | 1.1 | 3.9 | 0.9 | 3.1 |
| Phe (F) | 7.0 | 6.7 | 7.5 | 7.1 |
| Pro (P) | 4.8 | 6.3 | 4.2 | 4.0 |
| Ser (S) | 6.0 | 6.3 | 12.1 | 7.1 |
| Thr (T) | 4.2 | 4.3 | 3.7 | 4.0 |
| Trp (W) | 1.6 | 1.4 | 0.5 | 7.1 |
| Tyr (Y) | 2.5 | 2.0 | 1.4 | 6.7 |
| Val (V) | 4.8 | 7.5 | 5.6 | 0.9 |