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. 2022 May 6;80:104048. doi: 10.1016/j.ebiom.2022.104048

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© 2022 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig4 — COVID-19 convalescent patients mount mono-reactive SARS-CoV-2 S CD4+ T cell responses after vaccination. FEST assays were performed on PBMC samples from study participants prior to and following SARS-CoV-2 mRNA vaccination. The top 10 significantly (FDR <0.05) expanded SARS-CoV-2 specific CD4+ T cell clonotypes at post vaccine timepoint are shown from patients with history of COVID-19 at post-COVID, post-vaccine, and late post-vaccine (e, f, j) timepoints. Frequency among cultured CD4+ T cells is shown on y-axis and each replicate of the peptide pool or control conditions are shown on x-axis. Each TCR clonotypes is represented by a unique symbol.