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. 2022 Jan 19;3(2):100301. doi: 10.1016/j.xplc.2022.100301

Figure 5.

Figure 5

Reciprocal phosphorylation between SOBIR1 and BAK1

(A) Determination of the oligomerization states of SOBIR1-KD, BAK1-CD, and SOBIR1-KD incubated with BAK1-CD via SEC. The reaction mixture contained 30 μM SOBIR1-KD and 10 μM GST-BAK1-CD. Elution volumes of the protein standards are indicated.

(B) Western blotting analysis of the transphosphorylation of PP2Cα-dephosphorylated SOBIR1-KD by wild-type BAK1-CD. The reaction mixture contained 10 μM dSOBIR1-KD and 1 μM GST-BAK1-CD.

(C) Western blotting analysis of the transphosphorylation of BAK1-CDD434N by wild-type SOBIR1-KD. The reaction mixture contained 10 μM GST-BAK1-CDD434N and 1 μM SOBIR1-KD.