Skip to main content
. 2021 Dec 17;3(2):100269. doi: 10.1016/j.xplc.2021.100269

Figure 1.

Figure 1

Nkd1 inhibits PAMP-triggered ROS bursts in the plant nucleus.

(A) PAMP-triggered ROS bursts in N. benthamiana. The effect of transient expression of Nkd124-516-mCherry-3xHA on ROS bursts triggered by chitin (left) or flg22 (right). Plants expressing mCherry were used as the positive control. Total photon counts indicating plant-derived apoplastic ROS were collected over 30 (chitin) or 40 (flg22) min and are depicted as boxplots. Data are a pool of three independent experiments (∗p < 0.05, ∗∗p < 0.01, t test).

(B) Localization of NKd1 in N. benthamiana. Nkd124-516-mCherry-3xHA was co-expressed with GFP-NLS in epidermal cells, and localization was assessed by confocal microscopy. Nkd124-516-mCherry-3xHA localizes to the nucleus. Left panel: GFP fluorescence; middle panel: mCherry fluorescence; and right panel: bright field-GFP-mCherry merge. Scale bar, 50 μm.

(C) Subcellular localization of Nkd1 affects ROS burst-suppressive activity. Nkd124-516-NLS retains its ability to suppress flg22-triggered ROS bursts, whereas Nkd124-516-NES does not. Data are a pool of three independent experiments (∗p < 0.05, ∗∗p < 0.01, ANOVA, Tukey’s). For all panels, n = number of plants used for each group.