Figure 3.

Knockdown of SIX1 represses glycolysis in non-small cell lung cancer cells. (A) A549 cells were transfected with si-SIX1 (si-SIX1-1 and si-SIX1-2) or si-con, respectively. The mRNA level of SIX1 was detected by RT-qPCR. ***P<0.001 vs. si-con. (B) A549 cells were treated as in (A), and the protein level of SIX1 was measured by western blot analysis. The right panel shows the densitometric analysis of three independent experiments. *P<0.05 and **P<0.01 vs. si-con. (C) A549 cells were transfected with si-SIX1 or si-con respectively. Glucose uptake, lactate production and ATP level were measured by corresponding assays. **P<0.01 and ***P<0.001 vs. si-con. (D) A549 cells were treated as in (C), and the mRNA levels of PKM2, LDHA and HK2 were detected by RT-qPCR. **P<0.01 and ***P<0.001 vs. si-con. (E) A549 cells were treated as in (C), and the protein levels of PKM2, LDHA and HK2 were detected by western blot analysis. The right panel shows the densitometric analysis of three independent experiments. *P<0.05 and **P<0.01 vs. si-con. Tan IIA, Tanshinone IIA; SIX1, sine oculis homeobox homolog 1; PKM2, pyruvate kinase subtype M2; HK2, hexokinases 2; LDHA, lactate dehydrogenase A; RT-qPCR, reverse transcription-quantitative PCR; si-, small interfering; con, control.