Figure 6.

Tan IIA suppresses non-small cell lung cancer cell growth in vivo. A549 cells were injected into the right posterior ankle of the nude mice to establish xenograft tumors. From the eighth day, mice were intraperitoneally injected with Tan IIA (20 mg/kg) for 2 weeks. (A) Representative tumor sizes in each group of mice. (B) Tumor volumes were monitored by direct measurement. *P<0.05 and **P<0.01 vs. control group. (C) Xenograft tumor wet weight in each group of mice. **P<0.01 vs. control group. (D) The mRNA levels of SIX1, PKM2, LDHA and HK2 in xenograft tumor tissues were detected by reverse transcription-quantitative PCR. *P<0.05 and **P<0.01 vs. control group. (E) The protein levels of SIX1, PKM2, LDHA and HK2 in xenograft tumor tissues were detected by western blot analysis. The right panel shows the densitometric analysis of three independent experiments. *P<0.05 vs. control group. SIX1, sine oculis homeobox homolog 1; Tan IIA, Tanshinone IIAcon, control; PKM2, pyruvate kinase subtype M2; HK2, hexokinases 2; LDHA, lactate dehydrogenase A.