FIG. 5.

Antibiotic wash of 70S ribosomes. Hygromycin B or streptomycin (50 μM each) was added to 20 pmol of 70S ribosomes to induce the release of protein(s) from the ribosomes. The ribosomes were isolated by ultracentrifugation, and the supernatants were analyzed by immunoblotting as described in Materials and Methods. The data were scanned densitometrically, and the total RbbA released into the supernatant was taken as 100%. Column 1, control ribosomes (not washed) in a 5× excess; column 2, no antibiotic added; column 3, streptomycin wash; column 4, hygromycin B wash.