Summary of recently published reports on H₂S detection probes.

H2S probe	Reaction mechanism	Wavelength (nm)	Detection limit	Experimental subject	Detection method	Ref.
Cyclen-AF + Cu2+ (HSip-1)	Cu2+ quenches fluorescence	516	10 μM	HeLa cells	Fluorescence microscopy	69
SHS-M2	Azides to amines	464/545	0.4 μM	DJ-1 deficient astrocytes and brain slices	Two-photon microscopy	71
NanoBODIPY	Nucleophilic reactions	511/589	7 nM	Raw 264.7 macrophage cells	Confocal microscopy	76
Coumarin–merocyanine dyad (CPC)	Nucleophilic reactions	474/587	40 nM	HeLa cells	Confocal microscopy	77
Azide-functionalized O-methylrhodol (MeRho-Az)	Azides to amines	516	86 ± 7 nM	C6 cells and zebrafish	Light sheet fluorescence microscopy	80
Ruthenium(ii) complex-based luminescence probe (Ru-MDB)	Nucleophilic reactions	456/612	45 nM	Zebrafish and mice	Fluorescence microscopy and confocal microscopy	83
NIR-II@Si	Nucleophilic reactions	700/900	37 nM	HCT116 tumor mice	Fluorescence imaging	85
Si@BODPA	Nucleophilic reactions	780	53 nM	HCT116 tumor mice	Photoacoustic imaging	88
AzHD-LP	Azides to amines	600/700	91 nM	HCT116 tumor mice	Photoacoustic imaging	89