Fig. 8. Proposed docking pose of 2 and 22 in the active site of SPHK1 (PDB id: 3VZB) and SPHK2 (Homology model based on 3VZD) respectively. (A) Docking poses of 2 and resveratrol were overlapped with the X-ray pose of sphingosine in SPHK1. (B) Docking pose of 22 and SKI-II are shown in the substrate-binding site of SPHK2 homology model. Only hydrogen bonds with key residue (SPHK1: Asp178, SPHK2: Asp 308) were presented by red dotted. Carbon atoms are colored yellow (amino acid), magenta (2), cyan (22), silver (sphingosine), blue-green (resveratrol) and orange (SKI-II); nitrogen is blue; oxygen is red; hydrogen is light grey.