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. 2022 Mar 4;12(5):jkac053. doi: 10.1093/g3journal/jkac053

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© The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — High-resolution Hi-C of Neurospora crassa using the restriction enzyme MseI. a) Heatmap displaying the merged MseI in situ Hi-C dataset of LG II, either plotting raw read counts (above diagonal) or KR-corrected counts (below diagonal), at 20 kb bins. The magnified heatmap shows the intrachromosomal interaction between the left and right telomeres of LG II at 5 kb resolution. CenH3 (centromeric) and H3K9me3 (heterochromatic) ChIP-seq enrichment tracks presented above and to the left of each plot. Horizontal line at bottom of heatmap highlights region displayed in panel (c). b). Heatmap displaying the interactions between LG II and LG III in the merged MseI in situ Hi-C dataset at 20 kb. Magnified plots at 5 kb resolution show the interactions between the LG II/LG III centromeres (dashed lines) or the interactions between the left subtelomeres of LG II and LG III (box). c). Heatmaps of MseI (top) and DpnII (bottom) contacts at 5 kb bin resolution of the right arm of LG II (highlighted in A by horizontal line). Both triangle heatmaps were cropped and rotated 45° from square (reflective) heatmaps. Central IGV image displays tracks of MseI and DpnII restriction sites, H3K9me3 ChIP-seq enrichment and genes.