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. 2022 Apr 27;25(6):202. doi: 10.3892/mmr.2022.12718

Figure 1.

Figure 1.

Effects of circ_0067934 on proliferation of gastric cancer cells. (A) The expression level of circ_0067934 in gastric cancer cell lines was detected via RT-qPCR. ***P<0.001 vs. GES-1 cells. (B) Transfection efficiency of sh-circ_0067934 was measured via RT-qPCR. (C) Cell proliferation was evaluated using a Cell Counting Kit-8 assay. (D) EdU staining was used to assess cell proliferative capacity. (E) Western blotting was performed to detect the protein expression levels of Ki67 and PCNA. Data are presented as the mean ± SD. Original magnification, ×200. **P<0.01, ***P<0.001 vs. sh-NC. RT-qPCR, reverse transcription-quantitative PCR; circ, circular RNA; sh, short hairpin RNA; NC, negative control; PCNA, proliferating cell nuclear antigen; OD, optical density.