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. 2022 Apr 27;25(6):202. doi: 10.3892/mmr.2022.12718

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — miR-1301-3p targets KIF23. (A) The starBase database was used to predict the binding sequence of miR-1301-3p with KIF23. (B) The luciferase reporter assay predicted the binding of miR-1301-3p to KIF23. (C) The expression level of KIF23 in gastric cancer cell lines was detected via reverse transcription-quantitative PCR. (D) Western blotting was employed to examine the expression level of KIF23 in AGS cells after transfection of miR-1301-3p mimic. Data are expressed as the mean ± SD. ***P<0.001 vs. mimic-NC. NC, negative control; miR, microRNA; KIF23, kinesin family member 23; WT, wild-type; MUT, mutant.