FIG. 4.

Effects of cathelicidin peptides on the transepithelial resistance of primary cultures of human airway epithelia. Transepithelial resistance was measured at baseline and after 2.5 h (SMAP29, OV-1, and OV-2) or 4 h (LL37) of exposure to the peptides. The peptides were studied at concentrations of 1, 10, and 100 μg/ml. Transepithelial resistance values shown are normalized to 100% at zero hour. Following the peptide exposure measurement, the solution containing the peptide was removed and replaced with peptide-free solution for measurement of resistance at 24 h. Representative results are shown. The studies were performed three times in quadruplicate except for the LL37 studies, which were performed twice.