Fig. 2. miR-889-3p directly interacted with NEAT1 and E2F7. (A and B) The interaction of miR-889-3p with NEAT1 and E2F7 was predicted by Diana Tools and confirmed by the luciferase reporter system. (C and D) The enrichment of NEAT1 and E2F7 in the Me180 and HeLa cells transfected with miR-889-3p and miR-NC was evaluated by the RIP assay. (E and F) The correlation between miR-889-3p and NEAT1 (r = −0.8526, P < 0.0001) or E2F7 (r = −0.9525, P < 0.0001). (G) The correlation between NEAT1 and E2F7 (r = 0.8756, P < 0.0001). (H) The expression of miR-889-3p in the Me180 and HeLa cells transfected with pcDNA and NEAT1. (I) The E2F7 protein expression in the Me180 and HeLa cells transfected with miR-889-3p and miR-NC. (J) The E2F7 protein expression in the Me180 and HeLa cells transfected with si-NC, si-NEAT1, si-NEAT1+miR-NC and si-NEAT1+miR-889-3p. *P < 0.05.