Fig. 5. NEAT1 modulated cell progression by targeting the miR-889-3p/E2F7 axis through the PI3K/AKT pathway in CC. (A–C) The Me180 and HeLa cells were transfected with si-NC, si-NEAT1, si-NEAT1+in-miR-NC and si-NEAT1+in-miR-889-3p. (A) Protein expression of p-AKT, AKT, p-PI3K and PI3K in the transfected Me180 and HeLa cells. β-actin was used as an internal reference. (B and C) Relative protein expressions (p-AKT/AKT, p-PI3K/PI3K) in the transfected Me180 and HeLa cells. (D-F) The Me180 and HeLa cells were transfected with miR-NC, miR-889-3p, miR-889-3p+pcDNA and miR-889-3p+E2F7. (D) Protein expressions of p-AKT, AKT, p-PI3K and PI3K in transfected cells. (E and F) Relative protein expressions (p-AKT/AKT, p-PI3K/PI3K) in transfected cells. *P < 0.05.