Fig. 5. (a)–(c) Agarose gel electrophoresis for the mixtures of His–RLuc–EGFP–GB1 and QD, RLuc–His–EGFP–GB1 and QD, and His–EGFP–RLuc–GB1 and QD. The molar ratios of fused protein/QD are described in the images. The gel images were obtained by the NIR fluorescence of QDs at 830 nm. (d)–(f) Bioluminescence spectra of QD conjugates with His–RLuc–EGFP–GB1, RLuc–His–EGFP–GB1, and His–EGFP–RLuc–GB1 in the presence of CTZ. The molecular ratios of fused protein/QD were 14.‡Blue and green dotted lines show the spectral contribution from CTZ and EGFP, respectively.