Fig. 6. (a) Fluorescence autocorrelation curves for QDs, the mixture of QDs and His–EGFP–RLuc–GB1, and the mixture of QDs, His–EGFP–RLuc–GB1 and antibody (Ab) in PBS. (b) Diffusion time of QDs (1), the mixture of QDs and His–EGFP–RLuc–GB1 (2), and the mixture of QDs, His–EGFP–RLuc–GB1 and Ab (3). The values of diffusion time were obtained by using a single-component diffusion model.⁵³.