Fig. 8. (a) BRET and fluorescence (FL) images for the pellets of (a) KPL-4 cell (∼106 cells) and (b) A431 cell (∼106 cells) treated with QD–His–EGFP–RLuc–GB1 (none), normal human IgG/QD–His–EGFP–RLuc–GB1 (normal IgG), Herceptin/QD–His–EGFP–RLuc–GB1 (Herceptin), and Erbitux/QD–His–EGFP–RLuc–GB1 (Erbitux). The lower graphs show the emission intensity of BRET and FL for the above images. BRET and FL images were taken at 830 ± 20 nm and 530 ± 20 nm, respectively. Exposure time was 10 min both for BRET and FL imaging. (c) Western blotting analysis for the expression level of HER2 and EGFR in KPL-4 and A431 cells.
