Fluorescence quantum yield (QY) and lifetime (τ) of EGFP emission in His–EGFP–GB1, His–RLuc–EGFP–GB1, RLuc–His–EGFP–GB1, and His–EGFP–RLuc–GB1 protein.
Protein | QY | τ(ns) |
---|---|---|
His–EGFP–GB1 | 0.77 | 2.62 |
His–RLuc–EGFP–GB1 | 0.78 | 2.63 |
RLuc–His–EGFP–GB1 | 0.76 | 2.64 |
His–EGFP–RLuc–GB1 | 0.77 | 2.65 |