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. 2022 May 6;21:109. doi: 10.1186/s12943-022-01575-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — IGF2BP1 identifies m⁶A modified circMDK and improves the transcript stability of circMDK. A Bioinformatics analysis showed that there were m⁶A modification sites in circMDK. B The schematic diagram of MazF-PCR restriction endonuclease digestion. C The abundance of m⁶A modified circMDK in cells was detected by MazF-PCR. D The expression level of methylation-related proteins was verified by qRT-PCR. E The protein levels of IGF2BP1 in four paired HCC samples. pa: para-carcinoma tissues; ca: cancer tissues. F The qRT-PCR analysis of IGF2BP1 in 35 paired HCC samples. ***p < 0.001. G The schematic diagram of RIP assay. H Identification of the circMDK-protein complex pulled down by circMDK junction probe with protein extracts from Hep3B cells. The arrow indicates IGF2BP1. I RIP assays showing the association of IGF2BP1 with circMDK. Left, IP efficiency of IGF2BP1-antibody shown in western blotting. Right, relative enrichment representing RNA levels associated with IGF2BP1 relative to an input control. IgG antibody served as a control. **p < 0.01. J Transcript levels of IGF2BP1 and circMDK in negative control and si-IGF2BP1 HCC cells. **p < 0.01. K Reduction of circMDK RNA stability in IGF2BP1 knockdown HCC cells as compared with control. Cells were treated with Actinomycin D at the indicated time points. Error bars indicate SD. The t test was applied to analyze the statistical significance between two groups. **p < 0.01. L Correlation analysis revealed positive correlation between the levels of circMDK and IGF2BP1 mRNA in the tumorous tissues of the 35 HCC patients. Data are shown as mean ± SD of three independent experiments