FIG. 2.

RFLP analysis with Southern blotting with different chromosomal DNA samples. (A) Electrophoresis run. Lanes (K. pneumoniae strain tested and enzyme used for restriction, indicated by ⊥): 1, K251 ⊥ BamHI; 2, K273 ⊥ BamHI; 3, K534 ⊥ BamHI; 4, P111 (E. coli expressing TEM-1) ⊥ BamHI (negative control pattern); 5, K51 ⊥ EcoRI; 6, K51 ⊥ BamHI; 7, K51 ⊥ PstI; 8, K51 ⊥ NotI; 10, K48 ⊥ EcoRI; 11, K48 ⊥ BamHI; 12, K48 ⊥ PstI; 13, K48 ⊥ NotI; 14, K53 ⊥ EcoRI; 15, K53 ⊥ BamHI; 16, K53 ⊥ PstI; 17, K53 ⊥ NotI; 19, K75 ⊥ EcoRI; 20, K75 ⊥ BamHI; 21, K75 ⊥ PstI; 22, K75 ⊥ NotI; 9 and 18, unlabeled bacteriophage λ ⊥ HindIII (ladder marker). (B) Corresponding blot with labeled SHV-1 probe, as described in Materials and Methods.