TABLE 3.

DNA and deduced amino acid sequence alignments^a

Strain	% DNA homology	% Protein homology	Amino acid substitutionb	PCR result	Fragment size (kb) by RFLP analysis with EcoRI	β-Lactamase allelic variantc
TK75, TK136, TK167	100.00	100.00		+	8
TK175	100.00	100.00		+	8.5
TK198	99.91	100.00		+	8.5
K280 (Mzs)	99.54	100.00		+	8.5
K237 (Mzr)	100.00	100.00		+	8.5
K48 (Mzr)	98.80	99.65	Leu35Gln	+	7	SHV-31
K39	99.35	99.65	Leu35Gln	+	7	SHV-31
K117	99.53	100.00		+	8.5
K103, K218	99.36	99.65	Gly156Asp	+	8.5	SHV-27
K154	99.34	99.65	Tyr7Phe	+	8.5	SHV-28
K299	99.18	99.30	Ala126Val Gly156Asp	+	8.5	SHV-32
K8	99.63	99.65	Pro226Ser	+	8.5	SHV-33
K263d	90.92	90.91	Val22Ala, Tyr24Asp, Asn53Ser, Leu88Val, 284AlaAlaLeulleGluHisTrp290	−	8.5	LEN-2
SHV-1e	99.20	100.00				SHV-1
LEN-1f	89.95	87.76
OHIO-1g	94.88	94.41

^aMatches are expressed relative to the SHV-1_pMON38 sequence used in this study. Mz^r, mezlocillin resistant; Mz^s, mezlocillin susceptible. 

^bAmino acid sequence numbers according to Ambler et al. (1). 

^cSequence data for SHV-32, SHV-33, and LEN-2 will appear in the GenBank nucleotide sequence data under accession numbers AY037778, AY037779, and AY037780, respectively. Allele designations are reported at http://www.lahey.org/studies/webt.html. 

^dAmino acid substitutions for K263 were determined by comparison with the LEN-1 consensus sequence. 

^eSequence from GenBank (accession number AF148150). 

^fSequence from EMBL data bank (accession number X04515). 

^gSequence from EMBL data bank (accession number M33655).