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. 2022 May 6;22:181. doi: 10.1186/s12935-022-02592-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The effects of Lnc-LSAMP-1 on NSCLC biological phenotypes in vitro. a CCK8 assay was performed to determine the cell proliferation. b Cell proliferation assay in Incucyte zoom (Essen BioScience Co., Ltd., USA). c clone formation was performed to determine the cell proliferation. d, e Migration and invasion capacities determined by Transwell assays. f The flow cytometry was conducted to determine the cell apoptosis of A549 and PC-9 cells. g The flow cytometry was conducted to determine the cell cycle of A549 and PC-9 cells. The results from three independent experiments, showed as mean ± s.d, and scale bar: 100 μm; Significance was defined as p < 0.05 (*p < 0.05; **p < 0.01; ***p < 0.001)