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. 2022 May 5;22:125. doi: 10.1186/s12906-022-03610-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — RWF extract induced cell apoptosis of BPH-1 and WPMY-1 in a concentration-dependent manner. a BPH-1 and WPMY-1 cells were treated with the indicated concentration RWF extract for 72 h, followed by staining with FITC-Annexin and Propidium Iodide (PI). The cells treated with 5 μM cisplatin for 72 h were used as the positive controls. b, c Apoptotic ratio was determined by flow cytometry. Data were presented as mean ± SD; *** p < 0.001. d The expression levels of pro-apoptosis-related proteins in the presence of RWF extract by immunoblotting analysis in BPH-1 and WPMY-1 cells