TABLE 2.
Primers used in the study
| Primer | Sequence | Source | Gene or phage amplified | Positiona |
|---|---|---|---|---|
| #11 | GTCGAGGTCACGGCGTAC | M. tuberculosis | rpsL | 181–198 |
| #211 | CCCACCATTCAGCAGCTGGT | Mycobacteria | rpsL | |
| #212 | GTCGAGCGAACCGCGAATGA | Mycobacteria | rpsL | |
| #242 | CTACGGGAGGCAGCAGTGGG | M. smegmatis | rrs | 340–359 |
| #251 | GGCATCGCAGCCCTTTGTAC | M. smegmatis | rrs | 1,256–1,237 |
| #259 | TTTCACGAACAACGCGACAA | M. smegmatis | rrs | 599–580 |
| #261 | AAGGAGGTGATCCAGCCGCA | M. smegmatis | rrs | 1,539–1,520 |
| #264 | TGCACACAGGCCACAAGGGA | M. smegmatis | rrs | 1,072–1,052 |
| #285 | GAGAGTTTGATCCTGGCTCAG | M. smegmatis | rrs | 9–29 |
| #612 | GTAATACGACTCACTATAGGGC | pHRM3-GM | T7 | 625–646 |
| #651 | AATTAACCCTCACTAAAGGG | pHRM3-GM | T3 | 2,890–2,871 |
a
E. coli numbering. The positions of primers #211 and #212 can be found in reference 15.