TABLE 5.

Streptomycin MICs for rpsL and rrs mutant strains

Strain	MIC (μg/ml)
Parental strains
M. smegmatis mc2 155	<20
M. smegmatis rpsL3+	<20
M. smegmatis rrnB	<20
M. smegmatis rrnB rpsL3+	<20
rpsL mutant strains
rpsL42Lys→Arg	>200
rpsL42Lys→Asn	>200
rpsL42Lys→Thr	>200
rpsL42Lys→Met	>200
rpsL88Lys→Glu	200
rpsL88Lys→Arg	200
rrs mutant strains
rrs524G→C	>200
rrs526C→T	20–100
rrs522C→T	20–100
rrs523A→C	20–100
rrs mutant strains transformed with rrs wild-type gene
rrs524G→C::pMV361-H-rRNA2058G	<20
rrs526C→T::pMV361-H-rRNA2058G	<20
rrs523A→C::pMV361-H-rRNA2058G	<20
rrnB knockout strain transformed with different rrs allelesa
pMV361-H-rRNA2058bc	<20
pMV361-H-rRNA524C2058Gb	>200
pMV361-H-rRNA526T2058Gb	20–100
pMV361-H-rRNA523C2058Gc	20–100

^aMutants were tested after selection on medium containing hygromycin (50 μg/ml) plus streptomycin (20 μg/ml). 

^bStrain rrnB was transformed. 

^cStrain rrnB rpsL³⁺ was transformed.