Fig. 2. Effect of AS-IV on cell apoptosis. The HepG2 and Hep3B cell lines were conducted with AS-IV in different concentrations (25, 50 and 100 nmol l−1), respectively. (A) Cell apoptosis was detected by flow cytometry. (B and C) The expression levels of cleaved-caspase-3 and cleaved-caspase-9 protein and semi-quantitative analysis of the relative values normalized to β-actin. Data are showed as mean ± S.D, all individual experiments were presented at least three times. *p < 0.05 and **p < 0.01 compared with the control group.
